welcome to oneFAPvoice
- a positively charged Familial Adenomatous Polyposis community.- join today!
- login
Enhanced Cyclooxygenase-2 Expression in Sporadic and Familial Adenomatous Polyposis of the Human Colon
source: Scandinavian journal of gastroenterology
year: 2001
authors: Khan K N, Masferrer J L, Woerner B M, Soslow R, Koki A T
summary/abstract:BACKGROUND : The cyclooxygenase (COX) enzymes exist in two related but unique isoforms (COX-1 and COX-2) and catalyze the formation of prostaglandins (PGs). COX-1 is constitutively expressed, and is responsible for the synthesis of PGs necessary for gastroprotection and normal renal function. The COX-2 isoform is important in a variety of pathophysiological conditions such as inflammation and tumorigenesis. Numerous studies report that regular use of non-steroidal anti-inflammatory drugs (NSAIDs) can decrease the incidence of some tumor types, including gastrointestinal polyposis.
METHODS : In this study, we evaluated COX-1 and COX-2 expression in 30 polyps collected from 10 patients with familial adenomatous polyposis (FAP) and in 18 polyps collected from 18 patients with sporadic adenomatous polyposis (SAP) using COX-1 or COX-2 isoform-specific antibodies. All tissues were formalin-fixed and paraffin-embedded. Immunoreactivity was detected using tyramide signal amplification and evaluated utilizing an immunohistochemical scoring system.
RESULTS : COX-2 was minimally detected in the distant non-neoplastic epithelium, which also served as an internal negative control. In comparison, all polyps collected from SAP or FAP patients overexpressed COX-2 in the neoplastic epithelial cells (P < or = 0.002). Additionally, pronounced COX-2 expression was observed in the stromal cells underlying and adjacent to adenomatous lesions. COX-1 immunoreactivity was weak to mild throughout each tissue evaluated and did not change in the neoplastic or stromal cells of the polyps.
CONCLUSIONS : COX-2 expression is upregulated in the adenomatous epithelium of SAP and FAP, while the COX-1 isoform appears to be constitutively expressed at low levels in both neoplastic and non-neoplastic regions. The differential expression of COX-1 and COX-2 in these neoplasms suggests that COX-2 rather than COX-1 may play a role in adenoma formation and/or growth in cases of SAP and FAP in humans.
organization: Skokie Pharmacia Research and Developmentread more
expertly curated content related to this topic
-
Clinical and Genetic Characterization of Classical Forms of Familial Adenomatous Polyposis: A Spanish Population Stu...BACKGROUND : Classical familial adenomat...
-
Familial Adenomatous Polyposis: A Bibliography and Dictionary for Physicians, Patients, and Genome ResearchersThe book covers these subjects among oth...
-
APC: The Key to Colon Cancer (The APC Protein and Its Role in Controlling the Cell Cycle)Poster Presentation Abstract: Colorec...
-
Disease Severity and Genetic Pathways in Attenuated Familial Adenomatous Polyposis vary Greatly but Depend on the Si...BACKGROUND: Attenuated familial adenoma...
-
MutYH-Associated Colon Disease: Adenomatous Polyposis is Only One of the Possible Phenotypes; A Family Report and Li...AIMS AND BACKGROUND: The MutY human hom...
-
Familial Adenomatous PolyposisFamilial adenomatous polyposis (FAP) is ...
-
Molecular Targeting of 15-Lipoxygenase-1 (15-LOX-1) for Apoptosis Induction in Human Colorectal CancersPrimary Objective: - To determine whethe...